Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
The Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit is a reliable tool for the precise measurement of MMP-2 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing accurate and reproducible results, making it ideal for a wide range of research applications.MMP-2, also known as gelatinase A, is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in processes such as tissue remodeling, wound healing, and tumor metastasis. Abnormal MMP-2 levels have been linked to various diseases including cancer, cardiovascular conditions, and inflammatory disorders, making it a valuable biomarker for studying these pathological conditions and exploring potential therapeutic strategies.Overall, the Human MMP-2 ELISA Kit offers researchers a valuable tool for studying the role of MMP-2 in disease pathogenesis and for developing targeted interventions to modulate its activity.
Product Name:
Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
SKU:
HUES02480
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
