Description
Human/Mouse AKT Pathway Phosphorylation Array (18 targets) (SARB0017)
The Human/Mouse Akt Pathway Phosphorylation Array (SARB0017) is a cutting-edge tool for studying signaling pathways involved in cell growth, survival, and proliferation. This array allows for the simultaneous detection of phosphorylation levels of 18 key proteins in the Akt pathway, providing researchers with valuable insights into cellular signaling mechanisms.With the ability to analyze both human and mouse samples, this array is versatile and suitable for a wide range of experiments. Whether studying cancer biology, cell metabolism, or drug responses, the Human/Mouse Akt Pathway Phosphorylation Array offers a comprehensive view of Akt pathway activation in various biological contexts.
Designed for easy and efficient analysis, this array enables high-throughput screening of multiple phosphorylation events in a single experiment. By identifying changes in protein phosphorylation patterns, researchers can unravel the complex network of signaling cascades that regulate cellular functions. Gain a deeper understanding of Akt pathway activation with the Human/Mouse Akt Pathway Phosphorylation Array and uncover new possibilities for therapeutic interventions in diseases such as cancer and metabolic disorders.
| Product Sku: | SARB0017 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human / Mouse |
| Number of Targets Detected: | 18 |
| Gene Symbols: | AKT1, AKT1S1, BAD, CDKN1B, EIF4EBP1, GSK3A, GSK3B, MAPK1, MAPK3, MTOR|PDK1, PRKAA1, PTEN, RAF1, RPS6, RPS6KA1, RPS6KA3, RPS6KB1, TP53 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / AMPK Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| AMPKa (P-Thr172)
| BAD (P-Ser112)
| 4E-BP1 (P-Thr36)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
|
GSK3a (P-Ser21)
| GSK3b (P-Ser9)
| mTOR (P-Ser2448)
| p27 (P-Thr198)
| P53 (P-Ser15)
|
P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
| Raf-1 (Ser301)
|
RPS6 (P-Ser235/Ser236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
