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Human Phospho-Cyclin B1 (S126) and Total Cyclin B1 PharmaGenie ELISA Kit (SBRS1782)

SKU:
SBRS1782
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-Cyclin B1 S126 and Total Cyclin B1 PharmaGenie ELISA Kit SBRS1782
  • Human Phospho-Cyclin B1 S126 and Total Cyclin B1 PharmaGenie ELISA Kit SBRS1782
€820
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Description

Human Phospho-Cyclin B1 (S126) and Total Cyclin B1 PharmaGenie ELISA Kit (SBRS1782)

Product SKU:SBRS1782
Size:96T
Application:The antibody pair provided in this kit recognizes Human Cyclin B1 phosphorylated at site Serine-126 as well as total Cyclin B1.
Uniprot:P14635
Gene ID:891
Gene Names:CCNB1
Pathway:AMPK Signaling / Cell Cycle / DNA Damage
Synonyms:G2/mitotic-specific cyclin-B1
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-Cyclin B1 (S126) and Total Cyclin B1 PharmaGenie ELISA Kit (SBRS1782) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageJurkat cells were treated with Calyculin A and Pervanadate. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageHeLa cells were treated with Nocodazole at 37°C for 20 hours. Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA

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