Description
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Human Phosphorylation Pathway Profiling Array (55 targets) (SARB0063)
The Human Phosphorylation Pathway Profiling Array (SARB0063) is a powerful tool for researchers studying cellular signaling pathways involved in phosphorylation events. This array contains 55 key targets related to phosphorylation, allowing for comprehensive analysis of signaling cascades in a high-throughput manner.Each target on the array is carefully selected to provide insight into the phosphorylation status of crucial proteins involved in various signaling pathways. By using this array, researchers can profile the phosphorylation levels of multiple targets simultaneously, enabling a deeper understanding of how these pathways are regulated in different experimental conditions.
This array is compatible with human samples, making it a valuable resource for studies in cell signaling, cancer biology, and drug discovery. By utilizing the Human Phosphorylation Pathway Profiling Array, researchers can uncover new signaling nodes, identify potential therapeutic targets, and elucidate the mechanisms underlying various diseases and biological processes.
| Product Sku: | SARB0063 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 55 |
| Gene Symbols: | AKT1, AKT1S1, AMPK1, AND, ATF2, ATM, BAD, CDKN1B, CREB1, EGFR|EIF2S1, EIF4EBP1, FOS, GSK3A, GSK3B, HDAC2, HDAC4, HSPB1, JAK1, JAK2, JUN, MAP2K1, MAP2K3, MAP2K6, MAP3K7, MAPK14, MAPK8, MTOR, NFKBIA, PDPK1, PTEN, PTPN11, PTPN6, RAF1, RELA, RPS6, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KA5, RPS6KB1, SMAD1, SMAD2, SMAD4, SMAD5, SRC, STAT1, STAT2, STAT3, STAT5A, STAT6, TBK1, TP53, TYK2, ZAP70 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / TGF-beta Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4E-BP1 (P-Thr36)
| AKT (P-Ser473)
| AMPKa (P-Thr172)
| ATF2 (P-Thr69/71)
| ATM (P-Ser1981)
|
BAD (P-Ser112)
| C-Fos (P-Thr232)
| c-Jun (P-Ser73)
| CREB (P-Ser133)
| EGFR (P-Ser1070)
|
eIF-2a (P-Ser51)
| ERK1/2 (P-Thr202/P-Tyr204/P-Tyr185/P-Tyr187)
| GSK-3 alpha (P-Ser21)
| GSK-3 beta (P-Ser9)
| HDAC2 (P-Ser394)
|
HDAC4 (P-Ser632)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
| JAK1 (P-Tyr1022)
| JAK2 (P-Tyr1007/1008)
|
JNK (P-Thr183)
| MEK (P-Ser217/221)
| MKK3 (P-Ser189)
| MKK6 (P-Ser207) (MEK6)
| MSK1 (P-Ser376)
|
MSK2 (P-Ser360)
| mTOR (P-Ser2448)
| NF-kB (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
|
P53 (P-Ser15)
| P70S6K (P-Thr421/Ser424)
| PDK1 (P-Ser241)
| PRAS40 (P-Thr246)
| PTEN (P-Ser380)
|
Raf-1 (P-Ser301)
| RPS6 (P-Ser235/236)
| RSK1 (P-Ser380)
| RSK2 (P-Ser386)
| SHP-1 (P-Ser591)
|
SHP2 (P-Tyr542)
| SMAD1 (P-Ser463/465)
| SMAD2 (P-Ser245/250/255)
| SMAD4 (P-Thr277)
| SMAD5 (P-Ser463/465)
|
Src (P-Tyr419)
| STAT1 (P-Ser727)
| STAT2 (P-Tyr689)
| STAT3 (P-Tyr705)
| STAT5 (P-Tyr694)
|
STAT6 (P-Tyr641)
| TAK1 (P-Ser412)
| TBK1 (P-Ser172)
| TYK2 (P-Tyr1054)
| ZAP70 (P-Tyr292)
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Phosphorylation Pathway Profiling Array C55 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 500X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
