null

Human Rhodanese Recombinant Protein (RPPB2245)

SKU:
RPPB2245
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16762
Research Area:
Enzymes
€273
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human Rhodanese Recombinant Protein
Product Code:RPPB2245
Size:20µg
Species:Human
Target:Rhodanese
Synonyms:EC 2.8.1.1, TST, MGC19578, RDS, Thiosulfate sulfurtransferase, Rhodanese.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The Rhodanese protein solution contains 20mM Tris-HCl, pH-8 and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MVHQVLYRAL VSTKWLAESI RTGKLGPGLR VLDASWYSPG TREARKEYLE RHVPGASFFD IEECRDTASP YEMMLPSEAG FAEYVGRLGI SNHTHVVVYD GEHLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLDRS LLKTYEQVLE NLESKRFQLV DSRSQGRFLG TEPEPDAVGL DSGHIRGAVN MPFMDFLTED GFEKGPEELR ALFQTKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPESRVS QGKSEKA

Rhodanese is a mitochondrial matrix enzyme that is encoded by the nucleus. Rhodanese is involved in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. Rhodanese catalyzes the chemical reaction of thiosulfate & cyanide to sulfite & thiocyanate (detoxification). Rhodanese is part of the transferase family of proteins. Rhodanese includes two highly conservative domains, identified as rhodanese homology domains. In mammals, the majority of cyanide is converted to thiocyanate. Rhodanese has weak mercaptopyruvate sulfurtransferase activity.

Recombinant Human Rhodanese produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular mass of 35.6 kDa. Rhodanese is fused to a 20 amino acid His-Tag at N-terminus and purified by conventional chromatography techniques.

UniProt Protein Function:TST: Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity. Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
UniProt Protein Details:

Protein type:EC 2.8.1.1; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular space; mitochondrion

Molecular Function:5S rRNA binding

Biological Process: cyanate catabolic process; epithelial cell differentiation; rRNA transport

NCBI Summary:This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
UniProt Code:Q16762
NCBI GenInfo Identifier:3122965
NCBI Gene ID:7263
NCBI Accession:Q16762.4
UniProt Secondary Accession:Q16762,Q6IB06, B3KRM1,
UniProt Related Accession:Q16762
Molecular Weight:33kDa
NCBI Full Name:Thiosulfate sulfurtransferase
NCBI Synonym Full Names:thiosulfate sulfurtransferase
NCBI Official Symbol:TST��
NCBI Official Synonym Symbols:RDS��
NCBI Protein Information:thiosulfate sulfurtransferase
UniProt Protein Name:Thiosulfate sulfurtransferase
UniProt Synonym Protein Names:Rhodanese
Protein Family:Toxic shock syndrome toxin
UniProt Gene Name:TST��

Related Products