Description
Human Th1/Th2/Th17 Array (34 targets) (SARB0071)
The Human TH1/TH2/TH17 Array (34 Targets) (SARB0071) is a powerful tool for researchers studying immune responses and cytokine signaling pathways. This array allows for the simultaneous detection and quantification of 34 key cytokines, chemokines, and growth factors involved in TH1, TH2, and TH17 immune responses.With high sensitivity and specificity, this array provides valuable insights into the complex interplay of cytokines in immune-mediated diseases such as autoimmune disorders, inflammatory conditions, and cancer. Researchers can use this array to profile cytokine expression levels in various cell types and biological samples, helping to identify potential biomarkers and therapeutic targets.
The Human TH1/TH2/TH17 Array (34 Targets) is a reliable and efficient tool for exploring the dynamic nature of immune responses and uncovering novel insights into the molecular mechanisms underlying immune regulation. Whether studying the pathogenesis of diseases or evaluating the efficacy of immunomodulatory therapies, this array offers a comprehensive and high-throughput solution for cytokine profiling in research settings.
| Product Sku: | SARB0071 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 34 |
| Gene Symbols: | CCL20, CD40, CD40LG, CSF2, CSF3, IFNG, IFNL2, IL10, IL12A, IL12B|IL13, IL17A, IL17F, IL17RA, IL1B, IL1R1, IL1R2, IL2, IL21, IL21R, IL22, IL23A, IL4, IL5, IL6, IL6R, IL6ST, LTA, TGFB1, TGFB3, TNF, TNFRSF18, TNFRSF8, TNFSF11 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | NFkB Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
CD30 (TNFRSF8)
| CD40 Ligand (TNFSF5)
| CD40 (TNFRSF5)
| GCSF
| GITR (TNFRSF18)
|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-1 R1
| IL-1 R2
|
IL-2
| IL-4
| IL-5
| IL-6
| IL-6 R
|
IL-10
| IL-12 p40
| IL-12 p70
| IL-13
| IL-17A
|
IL-17F
| IL-17 RA
| IL-21
| IL-21 R
| IL-22
|
IL-23
| IL-28A (IFN-lambda 2)
| MIP-3 alpha (CCL20)
| gp130
| TGF beta 1
|
TGF beta 3
| TNF alpha
| TNF beta (TNFSF1B)
| TRANCE (TNFSF11)
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Th1/Th2/Th17 Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
