Description
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Mouse Apoptosis Array (38 targets) (SARB0073)
The Mouse Apoptosis Array (SARB0073) offered by AssayGenie is a comprehensive research tool designed for the simultaneous detection of 38 apoptosis-related targets in mouse samples. This array allows researchers to investigate various aspects of apoptosis, a fundamental biological process involved in programmed cell death.With a high specificity and sensitivity, this array provides valuable insights into the regulation of apoptosis in various biological systems. The targets included in this array cover a wide range of apoptotic pathways, including key proteins involved in the initiation, execution, and regulation of apoptosis.
The Mouse Apoptosis Array is ideal for studies focusing on cell death mechanisms, cancer research, and drug discovery. By enabling the simultaneous analysis of multiple targets, researchers can gain a more comprehensive understanding of apoptotic processes and identify potential therapeutic targets for various diseases.Whether studying apoptosis in normal physiological processes or pathological conditions, the Mouse Apoptosis Array offers a powerful tool for researchers seeking to unravel the complexity of cell death pathways. Trust AssayGenie for reliable and accurate results in your apoptosis research endeavors.
| Product Sku: | SARB0073 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 38 |
| Gene Symbols: | BAD, BAX, BCL2, BCL2L11, BCL2L2, BID, BIRC3, BIRC5, CASP3, CASP8|CD40, CD40LG, CDKN1A, DIABLO, FAS, FASLG, HSPA4, HSPB1, HSPD1, HTRA2, IGF1, IGF2, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, LTA, PER27, TNF, TNFRSF10B, TNFRSF1A, TNFRSF1B, TNFRSF21, TP53, XIAP |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / IGF Signaling / MAPK Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
bad
| bax
| bcl-2
| bcl-w
| BID
|
BIM
| Caspase-3
| Caspase-8
| CD40 (TNFRSF5)
| CD40 Ligand (TNFSF5)
|
cIAP-2
| Cytochrome C
| DR6 (TNFRSF21)
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
|
HSP27
| HSP60
| HSP70
| HTRA2
| IGFBP-1
|
IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-5
| IGFBP-6
|
IGF-1
| IGF-2
| p21
| p27/Kip1 (CDKN1B)
| p53
|
SMAC
| Survivin (BIRC5)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TNF alpha
|
TNF beta (TNFSF1B)
| TRAIL R2 (TNFRSF10B/DR5)
| XIAP
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
