Description
Mouse Apoptosis Signaling Pathway Array (17 targets) (SARB0074)
The Mouse Apoptosis Signaling Pathway Array (SARB0074) is a powerful tool for studying apoptosis-related proteins in mouse samples. This array includes 17 key targets involved in the apoptosis signaling pathway, allowing researchers to simultaneously detect and analyze multiple proteins in a single experiment.The array is designed for use in various research applications, including drug discovery, biomarker identification, and pathway analysis. Each target is carefully selected to provide comprehensive coverage of the apoptosis signaling pathway, offering valuable insights into the mechanisms of cell death and survival.
With its high sensitivity and specificity, the Mouse Apoptosis Signaling Pathway Array (SARB0074) delivers accurate and reliable results, making it an essential resource for researchers studying apoptosis and its role in disease development and progression. By using this array, researchers can gain a better understanding of the complex processes underlying apoptosis and identify potential therapeutic targets for treating a variety of diseases, including cancer and neurodegenerative disorders.
| Product Sku: | SARB0074 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 17 |
| Gene Symbols: | AKT1, ATM, BAD, CASP3, CASP7, CDKN1B, CHEK1, EIF2S1, HSPB1, MAP3K7|MAPK14, MAPK8, NFKBIA, RELA, SMAD2, TP53 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling / PKC Signaling / TGF-beta Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
AKT (P-Ser473)
| ATM (P-Ser1981)
| BAD (P-Ser112)
| Caspase-3 (P-Asp175)
| Caspase-7 (P-Asp198)
|
CHK1 (P-Ser296)
| eIF-2a (P-Ser52)
| ERK1/2 (P-Thr202)
| HSP27 (P-Ser82)
| IKBa (P-Ser32)
|
JNK (P-Thr183)
| NF-kB P65 (P-Ser536)
| p27 (P-Thr198)
| p38 (P-Thr180/Tyr182)
| P53 (P-Ser15)
|
SMAD2 (P-Ser245)
| TAK1 (P-Ser412)
| |||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Apoptosis Signaling Pathway Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
