The Mouse C5a (Complement Component 5a) ELISA Kit - High Sensitivity is a sophisticated tool designed for the precise and quantitative measurement of C5a levels in various biological samples from mouse sources. As a pivotal component of the complement system, C5a plays a critical role in inflammation, immune response modulation, and defense against pathogens. With exceptional sensitivity and specificity, this high-sensitivity ELISA kit allows researchers to delve into the intricate functions of C5a and its implications in inflammatory cascades, immunoregulation, and the pathogenesis of various immune-related disorders. Accurate quantification of C5a levels is crucial for unraveling the complexities of the complement system and its interactions with the immune system. Manufactured under stringent quality control standards by Assay Genie, the Mouse C5a ELISA Kit - High Sensitivity offers robust performance, ensuring reliable and reproducible results. Its ease of use makes it a valuable asset for researchers exploring the diverse roles of C5a in immune responses, inflammation, and disease processes in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
