Mouse CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit
The Mouse CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit is a highly reliable and accurate assay kit designed for the quantitative measurement of CD40L levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CD40L is a critical protein involved in the immune response, playing a key role in the activation of B cells and the regulation of T cell function.
Dysregulation of CD40L has been implicated in various autoimmune diseases and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse CD40L CLIA Kit provides researchers with a powerful tool for investigating the role of CD40L in immune and inflammatory processes, offering reliable data that can help advance our understanding of these complex biological pathways.
Product Name:
Mouse CD40L (Cluster of Differentiation 40 Ligand) CLIA Kit
SKU:
MOES00179
Target:
Mouse CD40L (Cluster of Differentiation 40 Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD40L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD40L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD40L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CD40L. You can calculate the concentration of Mouse CD40L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
92-107
104-119
Average (%)
96
99
110
1:4
Range (%)
86-97
97-108
100-118
Average (%)
92
103
107
1:8
Range (%)
100-116
103-119
96-112
Average (%)
107
110
104
1:16
Range (%)
98-110
95-112
104-118
Average (%)
104
103
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
95-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.82
271.7
961.15
96.56
292.17
1042.83
Standard deviation
11.8
22.28
95.06
10.08
32.81
69.04
C V (%)
12.58
8.2
9.89
10.44
11.23
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CD40L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CD40L in samples. No significant cross-reactivity or interference between Mouse CD40L and analogues was observed.
