The Mouse Chem (Chemerin) ELISA Kit is specifically designed for the precise measurement of chemerin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for various research purposes.Chemerin is a key protein involved in inflammatory responses and metabolism regulation. It plays a crucial role in conditions such as obesity, type 2 diabetes, and inflammatory diseases, making it a valuable biomarker for studying these diseases and exploring potential treatments. Overall, the Mouse Chem (Chemerin) ELISA Kit from Assay Genie provides an efficient and reliable tool for researchers to investigate the role of chemerin in physiological and pathological processes in mouse models.
Product Name:
Mouse CHEM (Chemerin) ELISA Kit
SKU:
MOES00636
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
