Description
Mouse Cytokine Array (48 targets) (SARB0083)
The Mouse Cytokine Array (48 Targets) provides a comprehensive tool for studying the levels of various cytokines in biological samples. With the ability to simultaneously detect multiple cytokines, this array offers a high-throughput and cost-effective solution for cytokine profiling in both research and clinical settings.Featuring 48 unique targets, including key cytokines involved in immune regulation, inflammation, and cell signaling, this array allows for a broad analysis of cytokine expression patterns. Each target is carefully selected for its relevance in various physiological and pathological conditions, making this array a valuable resource for studying cytokine dynamics in processes such as immune responses, infection, and inflammation.
Utilizing a sandwich ELISA format, this array is easy to use and provides reliable and reproducible results. With its sensitivity and specificity, researchers can confidently quantify and compare cytokine levels in different samples, aiding in the identification of potential biomarkers, drug targets, and therapeutic strategies.Overall, the Mouse Cytokine Array (48 Targets) is a versatile and powerful tool for investigating cytokine-mediated pathways and unraveling the complex interplay of cytokines in health and disease. Whether exploring basic research questions or developing clinical diagnostics, this array offers a comprehensive and efficient approach to cytokine analysis.
| Product Sku: | SARB0083 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 48 |
| Gene Symbols: | ACE, AGER, AREG, CCL21, CD27, CD36, CD40LG, CD70, CDH1, CHRD|CTF1, CTLA4, DCN, DKK1, EGF, ENG, EPGN, EREG, F11R, GAS1, GAS6, GZMB, HGF, IFNL2, IL11, IL17B, IL17F, IL1RL1, IL1RN, IL20, IL21, IL25, IL2RA, IL6R, LGALS1, MADCAM1, MFGE8, MME, PRL, PTX3, SLPI, SPINT1, TNFRSF13B, TNFRSF9, TNFSF12, TNFSF18, TNFSF9, TREM1 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| ACE
| ALK-1
| Amphiregulin
|
Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
| CD27 Ligand (TNFSF7)
| CD36 (SR-B3)
| CD40 Ligand (TNFSF5)
|
Chordin
| CTLA-4 (CD152)
| Decorin
| DKK-1
| E-Cadherin
|
EGF
| Endoglin (CD105)
| Epigen
| Epiregulin
| Galectin-1
|
Gas 1
| Gas 6
| GITR Ligand (TNFSF18)
| Granzyme B
| HAI-1
|
HGF
| IL-1 R4 (ST2)
| IL-11
| IL-17B
| IL-17E (IL-25)
|
IL-17F
| IL-1 RA (IL-1 F3)
| IL-2 R alpha
| IL-20
| IL-21
|
IL-28A (IFN-lambda 2)
| IL-6 R
| JAM-A (CD321/F11R)
| MAdCAM-1
| MFG-E8
|
Neprilysin
| Pentraxin-3 (TSG-14)
| Prolactin
| RAGE
| TACI (TNFRSF13B)
|
TREM-1
| TWEAK (TNFSF12)
| TWEAK R (TNFRSF12)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
