The Mouse GZMS-B (Granzyme B) CLIA Kit is a powerful tool designed for the precise measurement of granzyme B levels in mouse serum, plasma, and cell culture supernatants. With its advanced technology, this kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Granzyme B is a key enzyme involved in immune responses, particularly in the function of cytotoxic T cells and natural killer cells. Its activity is crucial in eliminating infected or transformed cells, contributing to immune defense against pathogens and cancer.
Detecting and quantifying granzyme B levels can provide valuable insights into immune system function and the development of immunotherapies.This kit is essential for studying immune responses, infectious diseases, cancer, and autoimmune disorders in mouse models. By accurately measuring granzyme B levels, researchers can gain a deeper understanding of immune mechanisms and potential therapeutic targets. Choose the Mouse GZMS-B CLIA Kit for reliable and precise granzyme B quantification in mouse samples.
Product Name:
Mouse Gzms-B (granzyme B) CLIA Kit
SKU:
MOES00312
Target:
Mouse Gzms-B (granzyme B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GzmB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GzmB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GzmB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GzmB. You can calculate the concentration of Mouse GzmB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
99-113
93-105
Average (%)
102
107
100
1:4
Range (%)
89-102
102-117
88-102
Average (%)
95
108
93
1:8
Range (%)
101-118
98-114
103-117
Average (%)
109
104
109
1:16
Range (%)
98-113
96-112
103-118
Average (%)
106
102
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
98
EDTA plasma (n=5)
99-112
106
Cell culture media (n=5)
92-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.63
76.83
181.61
22.39
84.37
195.82
Standard deviation
1.97
9.17
16.69
1.86
6.3
18.86
C V (%)
8.34
11.94
9.19
8.31
7.47
9.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GzmB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GzmB in samples. No significant cross-reactivity or interference between Mouse GzmB and analogues was observed.
