The Mouse IFN-gamma (Interferon-gamma) ELISA Kit is specifically designed for the accurate measurement of IFN-gamma levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.IFN-gamma is a key cytokine involved in the immune response, playing a crucial role in the activation of immune cells and inflammation. It is essential for the defense against viruses, bacteria, and tumors.
Abnormal levels of IFN-gamma have been linked to autoimmune diseases, infectious diseases, and cancer, making it a valuable biomarker for studying these conditions and developing potential treatments. With its reliable performance and ease of use, the Mouse IFN-gamma ELISA Kit is a valuable tool for researchers studying the immune system, inflammation, and related diseases in mouse models.
Product Name:
Mouse IFN-gamma (Interferon Gamma) ELISA Kit
SKU:
MOES00667
Target:
Mouse IFN-gamma (Interferon Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
86-98
89-102
Average (%)
93
91
95
1:4
Range (%)
97-111
87-98
94-105
Average (%)
103
92
100
1:8
Range (%)
97-113
82-98
99-110
Average (%)
104
89
104
1:16
Range (%)
96-111
82-92
93-107
Average (%)
101
87
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
96-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.5
96.5
497.2
53.3
95.5
533.3
Standard deviation
3.2
4.4
20.9
3.7
4.7
17.1
C V (%)
6.21
4.56
4.2
6.94
4.92
3.21
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed.
