The Mouse IFNα (Interferon Alpha) ELISA Kit is a cutting-edge assay meticulously crafted for the precise and quantitative detection of Interferon Alpha levels in a diverse range of biological samples. Interferon Alpha is a vital cytokine that plays a pivotal role in the body's immune response by combating viral infections and regulating various immune responses. This essential protein is a key player in the antiviral defense mechanism, influencing immune surveillance, immune cell activation, and immune regulation. Accurate measurement of Interferon Alpha levels is crucial for unraveling its multifaceted functions in immune signaling, antiviral immunity, and inflammatory processes. The Mouse IFNα ELISA Kit enables researchers to explore the intricate role of Interferon Alpha in immune modulation, viral infection, and autoimmune disorders, shedding light on the underlying mechanisms of immune responses and potential therapeutic strategies. With exceptional sensitivity and specificity, the Mouse IFNα ELISA Kit by Assay Genie ensures dependable and reproducible results, empowering researchers to delve into the complexities of immune regulation and antiviral defense mechanisms. Manufactured under stringent quality control standards, this kit guarantees robust performance and user-friendly protocols, making it an ideal tool for investigating the dynamic interplay of Interferon Alpha in immune function and disease pathology. Choose Assay Genie's IFNα ELISA Kit to advance your research and gain profound insights into the immune-modulatory functions of Interferon Alpha.
Product Name:
Mouse IFN alpha (Interferon Alpha) ELISA Kit
SKU:
AEES00247
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
