The Mouse IL1-alpha CLIA Kit is a cutting-edge assay designed for the precise measurement of interleukin-1 alpha (IL1-alpha) levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL1-alpha is a key pro-inflammatory cytokine that plays a vital role in the regulation of immune responses and inflammation. It is involved in various physiological processes, including the immune response, tissue repair, and cell proliferation.
Dysregulation of IL1-alpha has been linked to a wide range of inflammatory diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.With its advanced technology and reliable performance, the Mouse IL1-alpha CLIA Kit is an essential tool for researchers studying inflammatory diseases, immune responses, and cytokine signaling pathways in mouse models. Trust in this kit to provide accurate and insightful data to advance your research goals.
Product Name:
Mouse IL-1 alpha CLIA Kit
SKU:
MOES00033
Target:
Mouse IL-1 alpha
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-1α. You can calculate the concentration of Mouse IL-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
94-109
85-99
Average (%)
107
101
92
1:4
Range (%)
86-101
95-107
83-97
Average (%)
93
102
90
1:8
Range (%)
98-113
95-106
89-104
Average (%)
104
100
96
1:16
Range (%)
86-97
84-96
98-114
Average (%)
91
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.5
48.55
210.79
28.1
46.53
214.55
Standard deviation
2.34
4.9
16.1
3.3
4.04
13.13
C V (%)
8.83
10.09
7.64
11.74
8.68
6.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-1α in samples. No significant cross-reactivity or interference between Mouse IL-1α and analogues was observed.
