The Mouse INHBA (Inhibin Beta A) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of INHBA levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.INHBA, also known as Inhibin Beta A, is a critical protein involved in regulating cell growth and differentiation. It plays a crucial role in various physiological processes, including the development of reproductive tissues and the immune system.
Dysregulation of INHBA has been implicated in diseases such as cancer, autoimmune disorders, and infertility, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse INHBA CLIA Kit offers researchers a powerful tool for studying the role of INHBA in physiological and pathological processes, with the potential to uncover new insights and therapeutic targets.
Product Name:
Mouse INHbA (Inhibin Beta A) CLIA Kit
SKU:
MOES00384
Target:
Mouse INHbA (Inhibin Beta A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INHbA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INHbA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INHbA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INHbA. You can calculate the concentration of Mouse INHbA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
102-118
89-103
Average (%)
104
109
96
1:4
Range (%)
85-99
86-99
95-110
Average (%)
91
92
103
1:8
Range (%)
91-103
100-115
89-103
Average (%)
97
106
96
1:16
Range (%)
100-117
99-116
103-116
Average (%)
108
106
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
99-115
106
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.92
41.68
249.7
24.34
41.32
271.3
Standard deviation
3.03
3.35
23.42
2.32
3.8
26.8
C V (%)
12.67
8.04
9.38
9.53
9.2
9.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INHbA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INHbA in samples. No significant cross-reactivity or interference between Mouse INHbA and analogues was observed.
