The Mouse MMP-12 (Matrix Metalloproteinase-12) CLIA Kit is designed for the precise measurement of MMP-12 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.MMP-12 is a key enzyme involved in tissue remodeling and degradation, playing a crucial role in inflammatory processes and respiratory diseases. Its dysregulation has been linked to conditions such as chronic obstructive pulmonary disease (COPD) and asthma, making it a valuable biomarker for studying these diseases and exploring therapeutic interventions.
With its advanced technology and optimized protocols, the Mouse MMP-12 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying MMP-12 biology and its implications in disease pathogenesis. Trust in the precision and performance of this kit for your experimental needs.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-12. You can calculate the concentration of Mouse MMP-12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-104
87-102
90-104
Average (%)
99
94
96
1:4
Range (%)
98-111
101-116
89-105
Average (%)
105
109
96
1:8
Range (%)
95-108
102-114
94-105
Average (%)
100
108
99
1:16
Range (%)
83-98
102-117
95-107
Average (%)
90
109
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
97-115
105
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.54
215.43
999.18
112.84
218.23
935.05
Standard deviation
8.9
20.68
86.43
9.31
19.16
99.68
C V (%)
8.51
9.6
8.65
8.25
8.78
10.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-12 in samples. No significant cross-reactivity or interference between Mouse MMP-12 and analogues was observed.
