The Mouse MPIF2 (Myeloid Progenitor Inhibitory Factor 2) CLIA Kit is a powerful tool for the precise measurement of MPIF2 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MPIF2 is a key regulator of myeloid progenitor cell differentiation and function, playing a vital role in the immune response and inflammation.
Dysregulation of MPIF2 levels has been implicated in various diseases, making it a valuable biomarker for studying these conditions and developing novel therapeutic strategies.With its user-friendly protocol and reliable performance, the Mouse MPIF2 CLIA Kit is an indispensable asset for researchers investigating the role of MPIF2 in immune-related disorders and exploring its potential as a target for therapeutic intervention.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MPIF2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MPIF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MPIF2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MPIF2. You can calculate the concentration of Mouse MPIF2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-107
90-104
95-112
Average (%)
98
97
102
1:4
Range (%)
98-113
90-101
99-115
Average (%)
106
95
105
1:8
Range (%)
88-100
89-102
94-110
Average (%)
95
96
102
1:16
Range (%)
98-115
94-105
100-115
Average (%)
106
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
100
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.38
58.78
243.36
24.21
54.07
261.0
Standard deviation
1.88
6.66
18.25
3.15
5.99
17.7
C V (%)
8.04
11.33
7.5
13.01
11.08
6.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MPIF2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MPIF2 in samples. No significant cross-reactivity or interference between Mouse MPIF2 and analogues was observed.
