The Mouse NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit is specifically designed for the accurate detection of NGAL levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NGAL is a key protein involved in various biological processes, including inflammation and kidney injury. It serves as a biomarker for conditions such as acute kidney injury, sepsis, and certain cancers, making it an important target for research and potential therapeutic development.With the Mouse NGAL ELISA Kit, researchers can confidently measure NGAL levels in mouse samples, providing valuable insights into the role of this protein in disease pathogenesis and progression.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
