The Mouse RETN (Resistin) ELISA Kit is expertly designed to facilitate the precise and quantitative detection of Resistin levels across a diverse range of biological samples. Resistin, a crucial adipokine intricately linked to metabolic regulation and inflammation, plays a vital role in modulating insulin sensitivity, glucose metabolism, and inflammatory processes within the body. Accurate measurement of Resistin levels is fundamental for unraveling its impact on metabolic homeostasis, insulin resistance, and inflammatory responses. As an essential mediator in various metabolic and inflammatory pathways, understanding the role of Resistin in disease mechanisms and its potential as a therapeutic target is of paramount importance. The Mouse RETN ELISA Kit by Assay Genie empowers researchers with exceptional sensitivity and specificity, ensuring the delivery of reliable and reproducible results. Manufactured under stringent quality control standards, this ELISA kit offers robust performance and user-friendly protocols, making it an excellent choice for researchers investigating the intricate interplay between Resistin, metabolic health, and inflammatory conditions. Advance your research with the Mouse RETN ELISA Kit and uncover novel insights into the roles of Resistin in metabolic disorders and inflammatory diseases.
Product Name:
Mouse RETN (Resistin) ELISA Kit
SKU:
AEES00248
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
