Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit
The Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of S100A9 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.S100A9 is a key protein involved in inflammation, immune response, and calcium binding. It plays a crucial role in various physiological and pathological processes, making it a valuable biomarker for studying inflammatory diseases, cancer, and neurodegenerative disorders.
The Mouse S100A9 CLIA Kit is essential for researchers looking to study these conditions and develop potential therapeutic interventions.Order now and unlock the potential of your research with the Mouse S100A9 CLIA Kit from Assay Genie.
Product Name:
Mouse S100A9 (S100 Calcium Binding Protein A9) CLIA Kit
SKU:
MOES00609
Target:
Mouse S100A9 (S100 Calcium Binding Protein A9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse S100A9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse S100A9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse S100A9. You can calculate the concentration of Mouse S100A9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
95-109
92-106
Average (%)
92
101
97
1:4
Range (%)
91-105
84-99
91-103
Average (%)
99
91
97
1:8
Range (%)
92-104
89-101
86-100
Average (%)
97
94
91
1:16
Range (%)
100-118
90-101
91-105
Average (%)
107
96
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
96
EDTA plasma (n=5)
83-98
90
Cell culture media (n=5)
93-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.12
313.46
998.06
99.66
287.41
1040.15
Standard deviation
8.05
25.42
83.04
11.72
25.61
66.99
C V (%)
8.29
8.11
8.32
11.76
8.91
6.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse S100A9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse S100A9 in samples. No significant cross-reactivity or interference between Mouse S100A9 and analogues was observed.
