Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit
The Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit is a specialized assay designed for the precise measurement of thrombin-antithrombin complex levels in mouse serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results, making it an essential tool for researchers studying thrombotic disorders, coagulation processes, and inflammation in mouse models.The thrombin-antithrombin complex is an important marker of coagulation and hemostasis, reflecting the balance between thrombin generation and its inhibition by antithrombin. Dysregulation of this balance is implicated in various pathological conditions, including thrombosis, disseminated intravascular coagulation, and sepsis. The Mouse TAT ELISA Kit provides researchers with a valuable tool for investigating these conditions and evaluating potential therapeutic interventions.Order the Mouse TAT Thrombin-Antithrombin Complex ELISA Kit today to advance your research in thrombosis, coagulation, and inflammation in mouse models.
Product Name:
Mouse TAT (Thrombin-Antithrombin Complex) ELISA Kit
SKU:
MOES01545
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.