Description
Mouse RAGE/AGER ELISA Kit (High Sensitivity)
**** The Mouse AGER (Total Advanced Glycosylation End Product Specific Receptor) ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously designed for the precise quantification of the Total Advanced Glycosylation End Product Specific Receptor (AGER) levels in a variety of mouse biological samples. AGER is a vital receptor involved in mediating cellular responses to advanced glycosylation end products, playing a crucial role in inflammation, oxidative stress, and cellular signaling. By quantifying AGER levels with high sensitivity, researchers can delve into the intricate pathways where AGER exerts its regulatory functions. This high-sensitivity ELISA kit empowers researchers to unravel the complexities of AGER biology, facilitating a comprehensive understanding of its implications in diverse physiological and pathological processes.
AGER's role in mediating inflammatory responses, oxidative damage, and cell signaling pathways can be thoroughly investigated, offering valuable insights into its impact on various disease conditions and potential therapeutic strategies. The Mouse AGER ELISA Kit - High Sensitivity, created by Assay Genie, ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for robust research outcomes. With stringent quality control measures and optimized sensitivity, this kit delivers reliable performance and user-friendly protocols, making it an indispensable tool for in-depth investigations into AGER biology and its significance in mouse models.
| Product Name: | Mouse RAGE/AGER ELISA Kit (High Sensitivity) |
| Product Code: | AEES00606 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.78-50 ng/mL |
| Sensitivity: | 0.47 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse AGER in samples. No significant cross-reactivity or interference between Mouse AGER and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse AGER concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AGER, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AGER. You can calculate the concentration of Mouse AGER in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
