The Mouse IFN-gamma (Interferon Gamma) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously developed for the precise quantitative measurement of Interferon Gamma (IFN-gamma) levels within a variety of biological samples sourced from mice. IFN-gamma, a pivotal cytokine known for its essential role in modulating immune responses, has diverse functions in regulating immune surveillance, cellular differentiation, and inflammatory processes. It serves as a critical mediator in orchestrating immune defenses against infections, tumors, and autoimmune conditions through its intricate signaling pathways. Equipped with high sensitivity, this specialized ELISA kit empowers researchers to explore the intricate roles and diverse functions of IFN-gamma in immune regulation and host defense mechanisms within mouse models. By delivering exceptional accuracy and detection capabilities, this high-sensitivity kit provides a valuable tool for investigating the involvement of IFN-gamma in immune responses, inflammation, and disease pathogenesis. Focused on quality and performance, the Mouse IFN-gamma ELISA Kit - High Sensitivity from Assay Genie ensures dependable and reproducible results, facilitating detailed investigations into the roles of IFN-gamma in immune-related processes and disease models. Designed for user-friendly operation and robust performance, this kit is an excellent choice for researchers conducting studies in the fields of immunology and inflammatory diseases using mouse models.
Product Name:
Mouse IFN-gamma ELISA Kit (High Sensitivity)
SKU:
AEES00594
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.53 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
