| Kit Name: | OTC (Oxytetracycline) ELISA Kit |
| Kit Size: | 96 Assays |
| Test Principle: | This kit uses Competitive-ELISA as the method. It can detect Oxytetracycline (OTC) in samples, such as meat, raw milk, feed, etc. |
| Sensitivity: | 0.3 ppb (ng/mL) |
| Reaction Mode: | 25°C, 30 min˜15 min |
| Detection Limit: | Meat, Milk, Egg---6 ppb; Feed---400ppb |
| Cross Reactivity: | Oxytetracycline ---100%, Chlortetracycline --- 300%, Tetracycline---206%, Doxycycline --- 18.5% |
| Sample Recovery Rate: | 90%±30%. |
| Kits Components: | | Item | Specifications | | ELISA Microplate | 96 wells | | Standard Liquid | 1.5 mL each (0 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb, 24.3 ppb) | | Antibody Working Solution | 7 mL | | HRP Conjugate | 7 mL | | 20×Concentrated Sample Diluent | 50 mL | | Substrate Reagent A | 6 mL | | Substrate Reagent B | 6 mL | | Stop Solution | 6 mL | | 20×Concentrated Wash Buffer | 25 mL | | Plate Sealer | 3 pieces | | Sealed Bag | 1 piece | | Manual | 1 copy | |
| Other materials required but not supplied: | | Item | Specifications | | Instruments: | Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g). | | Micropipettor: | Single-channel (20-200 µL, 100-1000 µL), Multi-channel (300 µL). | | Reagents: | Trichloroacetic acid, Na2 Fe (CN)5 NO 2H2O, ZnSO4 7H2O. | |
| Storage And Expiry Date: | Store the kit at 2-8°C. Avoid freeze. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8°C. |
