The Phospho-eIF4EBP1-S65 Antibody (CABP0032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically recognizes the phosphorylated form of eIF4EBP1 at Serine 65, allowing for precise detection and analysis in various cell types.eIF4EBP1 is a crucial protein involved in the control of protein synthesis, particularly in response to cellular stress and growth signaling pathways. Phosphorylation of eIF4EBP1 at Serine 65 is known to regulate its activity, ultimately impacting global protein translation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-eIF4EBP1-S65 Antibody
SKU:
CABP0032
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RNSP V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BP-1, 4EBP1, 4E-BP1, PHAS-I, Phospho-eIF4EBP1-S65
Positive Sample:
293T
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
13kDa
Observed MW:
15-20kDa
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method
Affinity purification
Gene ID
1978
RRID
AB_2771072
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells using Phospho-eIF4EBP1-S65 Rabbit pAb (CABP0032) at 1:700 dilution. 293T cells were treated by λPP mixed solution (1ul) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
