The Phospho-PKC delta-Y311 Antibody (CABP0496) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications.PKCdelta is a member of the protein kinase C (PKC) family, known for its involvement in signaling pathways regulating cell proliferation, differentiation, and apoptosis. Phosphorylation of PKCdelta at tyrosine 311 has been linked to its activation and downstream signaling cascades, making it a valuable target for studies in cancer biology, cell signaling, and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-PKC delta-Y311 Antibody
SKU:
CABP0496
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VGIY Q
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Purification Method
Affinity purification
Gene ID
5580
RRID
AB_2771424
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U937 and Raw264.7 cells, using Phospho-PKC delta-Y311 Rabbit pAb (CABP0496) at 1:1000 dilution. U-937 cells were treated with PMA/TPA (200nM) for 30 minutes.RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
