The PP2A Catalytic alpha Antibody (CAB6702) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. RRID AB_2767286 Gene ID 5515 Swiss Prot Synonym RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; PP2A Catalytic α
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PP2A Catalytic alpha Antibody
SKU:
CAB6702
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCIPELISA
Recommended Dilution:
WB
1:1000 - 1:5000
IHC-P
1:50 - 1:200
IF
/
ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-500μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Chromosome, Cytoplasm, Nucleus, Centromere, Cytoskeleton, Spindle Pole.
Calculated MW:
36kDa
Observed MW:
36kDa
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. RRID AB_2767286 Gene ID 5515 Swiss Prot Synonym RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; PP2A Catalytic α
Purification Method:
Affinity purification
Gene ID:
5515
RRID:
AB_2767286
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PP2A Catalytic α Rabbit pAb (CAB6702) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PP2A Catalytic α Rabbit pAb (CAB6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PP2A Catalytic α Rabbit pAb (CAB6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using PP2A Catalytic α Rabbit pAb (CAB6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg PP2A Catalytic α antibody (CAB6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (CAB6702) at a dilution of 1:1000.
Immunoprecipitation analysis of 500 μg extracts of Rat brain cells using 3 μg PP2A Catalytic α antibody (CAB6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (CAB6702) at a dilution of 1:1000.
