The Phospho-PKC alpha-S657/Y658 Antibody (CABP0970) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and specifically recognizes the phosphorylated form of PRKCA at serine 657 and tyrosine 658 residues. PRKCA is a key enzyme involved in various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PRKCA at S657/Y658 has been linked to its activation and modulation of downstream signaling cascades. This antibody enables the detection and analysis of phosphorylated PRKCA in different cell types, providing valuable insights into its role in cellular signaling events.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PKC alpha-S657/Y658 Antibody
SKU:
CABP0970
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GFSY VN
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method
Affinity purification
Gene ID
5578
RRID
AB_2863868
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PKC alpha-S657/Y658 Rabbit pAb (CABP0970) at 1:1000 dilution. 293T cells were treated with CIP(20uL/400ul) at 37℃ for 1 hour. 293T cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
