The Phospho-Src-Y419 Monoclonal Antibody (CABP1027) is a high-quality antibody developed for reliable detection and analysis of target proteins. SRC is a tyrosine kinase that plays a key role in multiple cellular processes, including cell growth, proliferation, and differentiation. Phosphorylation of SRC at tyrosine 419 is a critical event that regulates its activity and downstream signaling.This antibody, raised in rabbits, is highly specific for the phosphorylated form of SRC at tyrosine 419 and has been validated for use in Western blot applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-Src-Y419 Monoclonal Antibody
SKU:
CABP1027
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1572
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NEYT A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
6714
RRID
AB_2863911
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Src-Y419 Rabbit mAb (CABP1027) at 1:1000 dilution. NIH/3T3 cells were treated with pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
