The Porcine Apolipoprotein B (APOB) ELISA Kit from Assay Genie is specifically designed for the accurate and sensitive detection of APOB levels in porcine samples. This kit is ideal for researchers studying lipid metabolism, cardiovascular diseases, and atherosclerosis in pigs. Apolipoprotein B is a key component of lipoproteins responsible for transporting cholesterol and other fats throughout the body. Elevated levels of APOB have been associated with an increased risk of cardiovascular diseases, making it a valuable biomarker for assessing cardiovascular health in pigs.
With a high level of precision and reliability, the Porcine APOB ELISA Kit offers researchers the confidence they need to obtain consistent and reproducible results. This kit is compatible with a variety of sample types, including serum and plasma, making it versatile and suitable for a wide range of research applications. Overall, the Porcine APOB ELISA Kit provides a powerful tool for studying lipid metabolism and cardiovascular health in pigs, offering valuable insights into the role of APOB in health and disease.
Product Name:
Porcine Apolipoprotein B/ApoB ELISA Kit
SKU:
PRFI00017
Reactivity:
Porcine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
1.875 ng/mL
Range:
3.125-200 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-104%
86-104%
85-98%
EDTA Plasma (n = 5)
82-99%
85-100%
90-101%
Heparin Plasma (n = 5)
80-95%
93-99%
87-98%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
95-100
97
EDTA Plasma (n = 5)
86-101
95
Heparin Plasma (n = 5)
85-97
91
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
