Description
Porcine T Cell Response Array (19 targets) (SARB0098)
The Porcine T-Cell Response Array (SARB0098) by Assay Genie is a comprehensive tool for studying the immune response in pigs. This array allows for the simultaneous detection of 19 different targets involved in T-cell activation and function. With a focus on porcine samples, researchers can easily analyze the expression levels of key molecules such as cytokines, chemokines, and receptors in response to various stimuli.This array is specifically designed for studying T-cell responses in pigs, making it an invaluable resource for immunologists, veterinarians, and researchers working on infectious diseases, vaccine development, and host-pathogen interactions in swine.
With its high sensitivity and specificity, the Porcine T-Cell Response Array provides valuable insights into the complex mechanisms underlying immune responses in pigs, ultimately contributing to the advancement of animal health and disease prevention strategies.
| Product Sku: | SARB0098 |
| Size: | 2, 4, or 8 |
| Species Detected: | Porcine |
| Number of Targets Detected: | 19 |
| Gene Symbols: | CSF2, IFNG, IL10, IL12A, IL13, IL15, IL17, IL17A, IL17F, IL1A|IL1B, IL1RN, IL21, IL22, IL28B, IL4, IL6, IRAP1, NCAM1, TGFB1, TNF, TNFA, TNFSF2 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3)
|
IL-10
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
IL-17F
| IL-21
| IL-22
| IL-28B
| IL-4
|
IL-6
| NCAM-1 (CD56)
| TGF beta 1
| TNF alpha
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Porcine T Cell Response Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
