Human CEA (Carcinoembryonic Antigen) Quickstep ELISA Kit
The QuickStep Human CEA (Carcinoembryonic Antigen) ELISA Kit is a reliable and accurate tool for the detection of CEA levels in human serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, providing robust and consistent results for a variety of research applications.CEA is a well-known biomarker for various types of cancer, particularly colorectal cancer, making it a valuable target for diagnostic and prognostic purposes. The QuickStep Human CEA ELISA Kit allows researchers to study CEA levels in different biological samples, aiding in the understanding of cancer progression and response to treatments.With its user-friendly protocol and precise detection capabilities, the QuickStep Human CEA ELISA Kit is an essential tool for cancer research, offering accurate and reliable results for the study of CEA in oncology and beyond.
Product Name:
Human CEA (Carcinoembryonic Antigen) Quickstep ELISA Kit
SKU:
QSES030
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.