The QuickStep Rat PG (Progestrone) ELISA Kit is specifically designed for the precise measurement of progestrone levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for various research applications.Progestrone is a key hormone in the reproductive system, playing a vital role in the menstrual cycle, pregnancy, and other physiological processes. Imbalances in progestrone levels can contribute to various reproductive disorders and complications, highlighting the importance of monitoring and understanding its levels in biological samples.
Whether studying reproductive health, endocrine disorders, or drug development, the QuickStep Rat PG ELISA Kit offers a reliable tool for researchers to investigate progestrone levels in rat samples effectively. Trust in this kit for consistent and precise results in your progestrone research studies.
Product Name:
QuickStep Rat Progesterone ELISA Kit
Product Code:
QSES054
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
7.01 pg/mL
Range:
15.63-1000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 10 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat Pg are added to the micro ELISA plate wells. Rat Pg in samples (or standards) competes with a fixed amount of Rat Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Rat Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
51.82
153.05
376.37
50.57
167.62
390.77
Standard deviation
3.26
7.61
17.39
2.91
8.55
19.58
CV (%)
6.30
4.97
4.62
5.76
5.10
5.01
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
91-101
96
EDTA plasma(n=8)
94-107
101
Samples were spiked with high concentrations of Rat Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
