The Rat APOB (Apolipoprotein B) CLIA Kit is a specialized assay designed for the accurate quantification of Apolipoprotein B levels in rat serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Apolipoprotein B is a critical protein involved in lipid metabolism and cholesterol transport, playing a key role in the development of cardiovascular diseases and metabolic disorders. By accurately measuring Apolipoprotein B levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.Overall, the Rat APOB CLIA Kit is an indispensable tool for studying lipid metabolism, cardiovascular health, and related diseases in rat models, providing researchers with reliable data to advance their scientific investigations.
Product Name:
Rat ApoB (Apolipoprotein B) CLIA Kit
SKU:
RTES00615
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
7.5 ng/mL
Detection range:
12.5-800 ng/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
