The Rat C3 (Complement Component 3) ELISA Kit is a cutting-edge assay tailored for the precise quantitation of Complement Component 3 levels within diverse biological samples collected from rat models. Complement Component 3 is a pivotal protein in the complement system, crucial for immune responses, pathogen clearance, and inflammatory processes. Accurate measurement of C3 levels is instrumental in elucidating the functionality of the complement system, evaluating immune activities, and deciphering the role of C3 in various disease conditions. This ELISA kit empowers researchers to explore the intricate dynamics of Complement Component 3, shedding light on its involvement in immune regulation, infectious diseases, autoimmune disorders, and inflammatory responses. By quantifying C3 levels, researchers can gain valuable insights into the immune system's functionality, disease pathogenesis, and potential therapeutic targets. Understanding the quantitative variations of C3 is fundamental in deciphering immune signaling pathways and developing targeted interventions for immune-related disorders. The Rat C3 ELISA Kit offered by Assay Genie is characterized by exceptional sensitivity, offering precise and reproducible quantification of C3 levels in rat samples. Manufactured under rigorous quality control standards, this kit ensures robust performance and user-friendly protocols, making it an indispensable tool for researchers investigating the intricate interplay between the complement system and immune responses in health and disease.
Product Name:
Rat C3 (Complement Component 3) ELISA Kit
SKU:
AEES00364
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
