The Rat EGF (Epidermal Growth Factor) ELISA Kit is a specialized assay designed to quantitatively detect the levels of Epidermal Growth Factor in various rat biological samples. Epidermal Growth Factor (EGF) is a critical signaling protein that plays a key role in cell proliferation, differentiation, and survival. It is an essential molecule involved in tissue development, wound healing, and various cellular processes. Accurate measurement of EGF levels using this ELISA kit can provide valuable insights into the role of EGF in cellular growth, tissue repair, and maintenance of epithelial tissues. Dysregulation of EGF has been associated with various pathological conditions, including cancer, inflammatory diseases, and impaired wound healing, making it a crucial target for research in cell biology and regenerative medicine. The Rat EGF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, this assay provides robust performance, delivering accurate quantitative data on EGF levels in biological samples. It is a valuable tool for researchers studying growth factor signaling pathways and their implications in health and disease.
Product Name:
Rat EGF (Epidermal Growth Factor) ELISA Kit
SKU:
AEES00395
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
