The Rat ET-1 (Endothelin-1) CLIA Kit is a reliable and accurate tool for measuring levels of endothelin-1 in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and precise results for a variety of research experiments.Endothelin-1 is a key molecule involved in regulating blood vessel constriction and is known to play a critical role in cardiovascular health. Abnormal levels of endothelin-1 have been linked to conditions such as hypertension, heart failure, and pulmonary diseases, highlighting its importance as a biomarker for studying and understanding these diseases. The Rat ET-1 CLIA Kit provides researchers with a valuable tool for investigating the role of endothelin-1 in various physiological processes and disease states, ultimately contributing to the development of novel therapeutic strategies.
Product Name:
Rat ET-1 (Endothelin 1) CLIA Kit
SKU:
RTES00100
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
