The RAT GL (Beta-Galactosidase) Beta-CLIA Kit is specially designed for the accurate measurement of beta-galactosidase levels in rat samples. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.Beta-galactosidase is an important enzyme involved in various cellular processes, making it a valuable biomarker for studying cell function and metabolism.
Its role in breaking down lactose and other glycosides also makes it a useful tool for analyzing gene expression and protein function in research studies.Overall, the RAT GL (Beta-Galactosidase) Beta-CLIA Kit is a reliable and efficient solution for researchers looking to quantitatively measure beta-galactosidase levels in rat samples, providing valuable insights into cellular activity and function.
Product Name:
Rat GL beta (Galactosidase, Beta) CLIA Kit
SKU:
RTES00219
Target:
Rat GL beta (Galactosidase, Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GLβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat GLβ. You can calculate the concentration of Rat GLβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
86-98
90-101
Average (%)
107
92
96
1:4
Range (%)
95-111
96-109
84-96
Average (%)
102
101
90
1:8
Range (%)
89-103
99-116
95-108
Average (%)
95
106
101
1:16
Range (%)
99-112
97-112
99-111
Average (%)
105
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
92-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.84
254.47
811.24
89.37
256.68
789.77
Standard deviation
10.05
29.93
70.9
11.15
27.23
89.17
C V (%)
10.71
11.76
8.74
12.48
10.61
11.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat GLβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat GLβ in samples. No significant cross-reactivity or interference between Rat GLβ and analogues was observed.
