Rat LBP (Lipolysaccharide Binding Protein) CLIA Kit
The Rat LBP (Lipolysaccharide Binding Protein) CLIA Kit is a specialized assay kit designed for the accurate measurement of lipolysaccharide binding protein levels in rat samples such as serum and plasma. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Lipolysaccharide binding protein is an important component of the immune response, playing a key role in the recognition and clearance of bacterial lipopolysaccharides.
Dysregulation of LBP levels has been associated with various inflammatory conditions and infections, making it a valuable biomarker for studying immune responses and developing new therapies.The Rat LBP CLIA Kit is easy to use and provides reliable results, making it an essential tool for researchers studying immune function, inflammation, and infectious diseases in rat models. Unlock new insights into the role of lipolysaccharide binding protein with this advanced assay kit from Assay Genie.
Product Name:
Rat LBP (Lipolysaccharide Binding Protein) CLIA Kit
SKU:
RTES00362
Target:
Rat LBP (Lipolysaccharide Binding Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat LBP. You can calculate the concentration of Rat LBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
85-99
90-105
Average (%)
100
91
97
1:4
Range (%)
99-112
91-102
95-109
Average (%)
107
97
101
1:8
Range (%)
92-103
88-100
97-108
Average (%)
98
94
102
1:16
Range (%)
86-100
88-99
86-101
Average (%)
92
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
98-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.83
8.88
0.94
2.64
9.62
Standard deviation
0.1
0.28
0.86
0.11
0.21
0.86
C V (%)
9.62
9.89
9.68
11.7
7.95
8.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat LBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat LBP in samples. No significant cross-reactivity or interference between Rat LBP and analogues was observed.
