The Rat SDF-1 alpha (Stromal Cell-Derived Factor 1 alpha) ELISA Kit is specially designed for the precise measurement of SDF-1 alpha levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.SDF-1 alpha is a critical chemokine involved in various biological processes, including stem cell homing, tissue regeneration, and immune response modulation. It plays a key role in inflammatory disorders, cardiovascular diseases, and cancer progression, making it a valuable biomarker for understanding these conditions and potentially identifying therapeutic targets.
With the Rat SDF-1 alpha ELISA Kit, researchers can uncover valuable insights into the role of SDF-1 alpha in disease pathology and therapeutic interventions, ultimately advancing our understanding of complex biological processes and guiding the development of novel treatments.
Rat SDF-1 alpha (Stromal Cell Derived Factor 1 alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDF-1α. You can calculate the concentration of Rat SDF-1α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
88-102
99-115
Average (%)
97
95
105
1:4
Range (%)
89-102
82-96
87-100
Average (%)
94
88
92
1:8
Range (%)
90-103
86-99
87-101
Average (%)
96
92
94
1:16
Range (%)
93-105
83-96
90-104
Average (%)
99
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.69
153.18
425.95
51.67
160.68
385.94
Standard deviation
3.09
8.42
15.67
3.36
9.13
12.12
C V (%)
5.98
5.5
3.68
6.5
5.68
3.14
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDF-1α in samples. No significant cross-reactivity or interference between Rat SDF-1α and analogues was observed.
