Rat SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
The Rat SDF-1 (Stromal Cell-Derived Factor 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of SDF-1 levels in rat samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.SDF-1 is a critical chemokine involved in various physiological processes, including stem cell homeostasis, tissue repair, and inflammation.
Its dysregulation has been implicated in diseases such as cancer, cardiovascular disorders, and autoimmune conditions, highlighting its importance as a potential biomarker for studying and developing therapies for these conditions.Overall, the Rat SDF-1 CLIA Kit offers researchers a powerful tool to investigate the role of SDF-1 in health and disease, providing valuable insights into its mechanisms and potential therapeutic interventions.
Product Name:
Rat SDF-1 (Stromal Cell Derived Factor 1) CLIA Kit
SKU:
RTES00527
Target:
Rat SDF-1 (Stromal Cell Derived Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat SDF-1. You can calculate the concentration of Rat SDF-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
94-106
102-114
Average (%)
97
100
107
1:4
Range (%)
97-113
87-98
88-102
Average (%)
105
93
96
1:8
Range (%)
92-109
87-99
100-116
Average (%)
100
93
108
1:16
Range (%)
91-105
99-116
100-114
Average (%)
98
107
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.57
263.48
715.99
105.95
286.45
650.36
Standard deviation
9.84
30.41
80.33
12.06
32.8
51.38
C V (%)
8.9
11.54
11.22
11.38
11.45
7.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat SDF-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat SDF-1 in samples. No significant cross-reactivity or interference between Rat SDF-1 and analogues was observed.
