Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
The Rat VCAM-1 (Vascular Cell Adhesion Molecule-1) ELISA Kit is specifically designed for the accurate measurement of VCAM-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.VCAM-1 is a key protein involved in cell adhesion and plays a critical role in inflammatory responses and immune cell recruitment. Elevated levels of VCAM-1 have been linked to various inflammatory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targets.
With its reliable performance and ease of use, the Rat VCAM-1 ELISA Kit is essential for researchers striving to understand the role of VCAM-1 in disease pathogenesis and developing novel interventions. Purchase this kit today to advance your research in the field of inflammation and vascular biology.
Product Name:
Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
SKU:
RTES00881
Target:
Rat VCAM-1 (Vascuolar Cell Adhesion Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM-1/CD106. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat VCAM-1/CD106. You can calculate the concentration of Rat VCAM-1/CD106 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
98-114
88-100
Average (%)
94
106
93
1:4
Range (%)
96-108
82-92
91-105
Average (%)
101
88
98
1:8
Range (%)
99-114
83-94
96-109
Average (%)
106
88
103
1:16
Range (%)
101-117
85-98
94-108
Average (%)
107
92
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
100
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.8
71.2
345.3
42.1
77.8
375.5
Standard deviation
2.5
3.2
15.9
2.7
3.5
13.5
C V (%)
6.28
4.49
4.6
6.41
4.5
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat VCAM-1/CD106 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat VCAM-1/CD106 in samples. No significant cross-reactivity or interference between Rat VCAM-1/CD106 and analogues was observed.
