Tumor necrosis factor receptor superfamily member 9(TNFRSF9); also known as CD137 and 4-1BB; is an inducible T cell surface protein belonging to the tumor necrosis factor receptor superfamily. It is a single-pass type I membrane protein which contains 4 TNFR-Cys repeats. The human and mouse proteins share 60% amino acid sequence identity. CD137 is expressed by mesenchymal cells; including endothelial cells; chondrocytes; and cells of the central nervous system. CD137 is also broadly expressed by cells of the human immune system; is broadly expressed by cells of the human immune system; including activated CD8+ and CD4+ T cells; activated natural killer (NK) cells; follicular dendritic cells (FDCs) and monocytes. CD137 has diverse roles in the immune response; the one key function is to promote the survival of both T cells and dendritic cells by binding the cognate ligand CD137L (4-1BBL).
Immobilized Human 4-1BBL-His(Cat: PKSH032023) at 0.03µg/ml(100 ?l/well) can bind Human 4-1BB-Fc. The ED50 of Human 4-1BB-Fc is 6.86 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human 4-1BB ligand receptor is produced by our Mammalian expression system and the target gene encoding Leu24-Gln186 is expressed with a Fc tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
44.2 kDa
AP Mol Mass:
58 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
