Interleukin-4 (IL-4) is a pleiotropic cytokine that controls a variety of T and B cell activities, including cell growth, survival, and gene expression. T cells, mast cells, and bone marrow stromal cells all make IL-4. IL-4 modulates the differentiation of naive CD4+ T cells into helper Th2 cells; characterized by a cytokine-release profile that includes IL-4, IL-5, IL-6, and IL-13; which promotes a humoral immune response. IL-4 has a major influence on the switching of immunoglobulins to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells is linked to excessive IgE synthesis and an allergic reaction. Human Interleukin-4/IL-4 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-4/IL-4 Recombinant Protein (RPES2502)
Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 7-27 pg/mL.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-4 is produced by our E.coli expression system and the target gene encoding His25-Ser153 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.1 kDa
AP Mol Mass:
13-14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
