Description
Recombinant Human uPA/PLAU Protein
The Recombinant Human uPA/PLAU Protein is a biologically active recombinant protein that plays a significant role in various cellular processes and signaling pathways in human biology. This protein is widely employed in immunological research, cell biology studies, protein-protein interaction analyses, and therapeutic development, providing researchers with a reliable tool for investigating uPA/PLAU function and its implications in health and disease.
This product (SKU: RPCB0227) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 49.36 kDa with an observed molecular weight of 55 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
| High Purity by Affinity Chromatography | |
| Mammalian & Bacterial Expression Systems | |
| High lot-to-lot consistency via strict QC |
| Product Name: | Recombinant Human uPA/PLAU Protein |
| SKU: | RPCB0227 |
| Size: | 10 μg , 20 μg , 50 μg , 100 μg |
| Reactivity: | Human |
| Synonyms: | PLAU, ATF, BDPLT5, QPD, UPA, URK, u-PA, urokinase |
| Tag: | C-His |
| Expression Host: | HEK293 cells |
| Calculated MW: | 49.36 kDa |
| Observed MW: | 55 kDa |
| Gene ID: | 5328 |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human uPA/PLAU Protein (RP00146LQ), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 0.1 EU/μg of the protein by LAL method. |
| Purity: | ≥ 95 % as determined by SDS-PAGE. |
| Formulation: | Supplied as a 0.22 μm filtered solution in 20mM HEPES, 150mM NaCl, 2mM CaCl, 10% Glycerol, pH 7.5Contact us for customized product form or formulation. |
| Bio-Activity: | Measured by its binding ability in a functional ELISA. Immobilized Human uPAR Protein at 1 μg/mL (100 μL/well) can bind PLAU with a linear range of 0.031-1.014 ng/mL. |
| Storage: | Store at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Plasminogen activator, urokinase, also known as PLAU and uPA, is a serine protease which converts plasminogen to plasmin, a broad-spectrum protease active on extracellular matrix (ECM) components. It is involved in complement activation, cell migration, wound healing, and generation of localized extracellular proteolysis during tissue remodelling, pro-hormone conversion, carcinogenesis and neoplasia. uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction). The role of uPA is not only does it as a kind of enzyme, but also is breast cancer, stomach cancer, colon cancer, bladder cancer, ovarian cancer, brain, and endometrial cancer markers for a strong invasion and metastasis.Because of the causal involvment of uPA in cancer invasion and metastasis, the blockade of uPA interactions and activity with specific inhibitors is of interest for novel strategies in cancer therapy.
 | Recombinant Human uPA/PLAU Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue. |
 | Immobilized Recombinant Human uPAR Protein at 1 μg/mL (100 μL/well) can bind PLAU with a linear range of 0.031-1.014 ng/mL. |
