Human Uteroglobin/SCGB1A1 Recombinant Protein (RPES0799)

SKU:
RPES0799
Product type:
Recombinant Protein
Host species:
HEK293 Cells
Reactivity:
Human
€399

Description

ELISA Kit Technical Manual

Recombinant Human Uteroglobin/SCGB1A1 Protein (His Tag)(Active)

Background:Uteroglobin (UG), also known as Secretoglobin 1A member 1 (SCGB1A1), Blastokinin, Clara cell secretor protein (CCSP) or Clara cell-specific 10-kDa protein (CC10), is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is mainly expressed in lung, with anti-inflammatory/immunomodulatory properties. Previous in vitro studies demonstrated that CCAAT/enhancer-binding proteins (C/EBPs) are the major transcription factors for the regulation of SCGB1A1 gene expression, whereas FOXA1 had a minimum effect on the transcription. Uteroglobin is a multifunctional protein with antiinflammatory/immunomodulatory properties. Uteroglobin inhibits soluble phospholipase A(2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, Uteroglobin manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities. The tissue-specific expression of the Uteroglobin gene is regulated by several steroid hormones, although a nonsteroid hormone, prolactin, further augments its expression in the uterus. Based on its anti-inflammatory and antiallergic properties, Uteroglobin is a potential drug target. The mechanism of Uteroglobin action is likely to be even more complex as it also functions via a putative receptor-mediated pathway.
Name:Recombinant Human Uteroglobin/SCGB1A1 Protein (His Tag)(Active)
Size:50µg
Assay Genie SKU:RPES0799
Synonyms:Uteroglobin; Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; Secretoglobin family 1A member 1; Urinary protein 1; UP; UP1; Urine protein 1; SCGB1A1; CCSP; UGB
Species:Human
Expression host:HEK293 Cells
Sequence:Met 1-Asn 91
Accession:P11684
Mol Mass:9.2 kDa
AP Mol Mass:9.2 kDa
Tag:C-His
Bio Activity:Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cell line. When 5 x 10E4cells/well are added to human SCGB1A1 coated plates (2 ?g/ml and 100 ?l/well), approximately >30% will adhere after one hour at 37 ?.
Purity:> 97 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile PBS, pH 7.4
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:SCGB1A1: Binds phosphatidylcholine, phosphatidylinositol, polychlorinated biphenyls (PCB) and weakly progesterone, potent inhibitor of phospholipase A2. Belongs to the secretoglobin family.
UniProt Protein Details:

Protein type:Secreted; Cell cycle regulation; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: extracellular space; rough endoplasmic reticulum; nuclear envelope; secretory granule

Molecular Function:protein binding; phospholipase A2 inhibitor activity

Biological Process: response to drug; negative regulation of interleukin-4 production; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; negative regulation of transcription from RNA polymerase II promoter; signal transduction; regulation of mRNA stability; response to xenobiotic stimulus; response to silicon dioxide; negative regulation of interleukin3 production; negative regulation of T cell proliferation; response to ozone; negative regulation of interferon-gamma production; response to cytokine stimulus; negative regulation of catalytic activity; regulation of inflammatory response; negative regulation of interleukin-5 production; embryo implantation

Disease: Asthma, Susceptibility To

NCBI Summary:This gene encodes a member of the secretoglobin family of small secreted proteins. The encoded protein has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma. [provided by RefSeq, May 2010]
UniProt Code:P11684
NCBI GenInfo Identifier:112672
NCBI Gene ID:7356
NCBI Accession:P11684.1
UniProt Related Accession:P11684
Molecular Weight:16kDa
NCBI Full Name:Uteroglobin
NCBI Synonym Full Names:secretoglobin family 1A member 1
NCBI Official Symbol:SCGB1A1  
NCBI Official Synonym Symbols:UGB; UP1; CC10; CC16; CCSP; UP; CCPBP  
NCBI Protein Information:uteroglobin
UniProt Protein Name:Uteroglobin
UniProt Synonym Protein Names:Clara cell phospholipid-binding protein; CCPBP; Clara cells 10 kDa secretory protein; CC10; Secretoglobin family 1A member 1; Urinary protein 1; UP; UP1; Urine protein 1
Protein Family:Uteroglobin
UniProt Gene Name:SCGB1A1  
UniProt Entry Name:UTER_HUMAN
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