The ST-5-HT (Serotonin, 5-Hydroxytryptamine) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of serotonin levels in various samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.Serotonin, also known as 5-hydroxytryptamine, is a neurotransmitter that plays a crucial role in regulating mood, behavior, and sleep. Imbalances in serotonin levels have been linked to various psychiatric disorders, including depression, anxiety, and schizophrenia.
This ELISA kit allows for the precise quantification of serotonin levels, making it an essential tool for studying the role of serotonin in health and disease.Overall, the ST-5-HT ELISA Kit is a valuable resource for researchers studying the intricate roles of serotonin in various physiological and pathological processes, providing accurate and consistent results for their studies.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ST/5-HT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
86-99
97-112
Average (%)
97
92
104
1:4
Range (%)
83-96
92-103
96-108
Average (%)
90
98
102
1:8
Range (%)
84-97
86-99
93-108
Average (%)
90
92
101
1:16
Range (%)
84-97
94-107
94-109
Average (%)
90
99
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
91-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.2
141.3
362.7
54.7
132.0
351.0
Standard deviation
3.5
5.9
15.6
3.1
6.5
16.1
C V (%)
6.84
4.18
4.3
5.67
4.92
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ST/5-HT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ST/5-HT in samples. No significant cross-reactivity or interference between ST/5-HT and analogues was observed.
