The ST-5-HT (Serotonin, 5-Hydroxytryptamine) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of serotonin levels in various samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.Serotonin, also known as 5-hydroxytryptamine, is a neurotransmitter that plays a crucial role in regulating mood, behavior, and sleep. Imbalances in serotonin levels have been linked to various psychiatric disorders, including depression, anxiety, and schizophrenia. This ELISA kit allows for the precise quantification of serotonin levels, making it an essential tool for studying the role of serotonin in health and disease.Overall, the ST-5-HT ELISA Kit is a valuable resource for researchers studying the intricate roles of serotonin in various physiological and pathological processes, providing accurate and consistent results for their studies.
Product Name:
ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit
SKU:
UNES00023
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
