The TIMP1 Antibody (CAB1389) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.TIMP1 is a key player in the proteolytic balance within tissues, acting as an inhibitor of matrix metalloproteinases (MMPs) and influencing various physiological and pathological processes such as tissue repair, inflammation, and tumor progression. By targeting TIMP1 with this antibody, researchers can detect and analyze its expression in different cell types, providing valuable insights into its function and potential therapeutic implications in conditions like cancer, fibrosis, and cardiovascular diseases.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIMP1 Antibody
SKU:
CAB1389
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EPA, EPO, HCI, CLGI, TIMP, TIMP-1, TIMP1
Positive Sample:
HeLa, Rat uterus
Cellular Localization:
Secreted.
Calculated MW:
23kDa
Observed MW:
28kDa
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Purification Method
Affinity purification
Gene ID
7076
RRID
AB_2760743
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TIMP1 Rabbit pAb (CAB1389) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using TIMP1 Rabbit pAb (CAB1389) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
